1 Industry Chain Analysis of Oligonucleotide Pool Library
The upstream of the Oligonucleotide Pool Library is the supply of raw materials such as phosphoramidites. The midstream is the Oligonucleotide Pool Library synthesis enterprise. The downstream applications include Target Capture, CRISPR/Cas9 Designs, Gene Synthesis, and Library Preparation.
2 Key Raw Materials
Nucleoside Phosphoramidites were first described in 1981. Phosphoramidites are modified nucleosides and are a standard chemical used in modern DNA synthesis. Since the introduction of phosphoramidite chemistry for DNA synthesis by Caruthers in 1981, it has become the golden standard for the synthesis of oligonucleotides. The key step in this synthetic approach for DNA synthesis is the reaction of the nucleoside phosphoramidite building block, with the terminal 5′-OH of the oligonucleotide. Through further development, automated oligonucleotide synthesis on solid support has evolved to the classical 4-step synthesis cycle. Today, many modified versions of phosphoramidites exist, each with varying properties optimized for specific DNA synthesis processes. With the commercialization of automated DNA synthesizers, chemical oligonucleotide synthesis has become a commodity for many research institutes, and several companies have specialized in custom oligonucleotide synthesis.
3 Key Raw Materials Price Trend
The costs and prices of gene synthesis continue to decrease, and the price of phosphoramidite synthesis is decreasing every year. But the affordability of custom DNA synthesis has not kept pace and the cost remains relatively high. For the past several years, there has been a general trend toward gene synthesis costs falling to $0.01 per bp, as opposed to less than the $1–2 per Gigabase offered by the latest next-generation sequencing tools.
Traditionally, oligonucleotides have been synthesized by solid‐phase phosphoramidite chemistry. This column‐based synthesis generates up to 200 mers with error rates of 1 in 200, and yields of 10 to 100 nmol per product for a cost of 0.05–1 USD per base dependent on the length and concentration yield. These individually synthesized oligonucleotides are then routinely further used for the synthesis of gene‐length DNA fragments using different PCR‐based methods.
To increase throughput and decrease the cost of oligonucleotide synthesis, several technologies have been developed over the last three decades to synthesize oligonucleotides in spatially decoupled microarrays, lowering costs by several orders of magnitude. In general, the costs of microchip-based oligo synthesis are 2–4 orders of magnitude cheaper than the column-based oligo synthesis. The cost per nucleotide is between $0.00001 and $0.001.
4 Key Suppliers of Raw Materials
Several key suppliers occupy an important position in the global market for the supply of raw materials for oligonucleotide pool library. These suppliers not only provide high-quality phosphoramidite raw materials necessary for the synthesis of oligonucleotides, but also drive the entire industry through continuous technological innovation and product development. Their presence ensures the stability of the supply chain from the research laboratory to commercial production, and plays an indispensable role in maintaining the industry’s competitiveness and promoting scientific and technological progress. The global network coverage and specialized services of these suppliers provide a solid foundation for the production of oligonucleotide pool library and reflect the growing demand for high-quality raw materials in this field. With the rapid development of fields such as synthetic biology and precision medicine, these suppliers play an increasingly critical role in supporting innovation and meeting the demands of complex markets.
Table Key Raw Materials Suppliers Analysis
Raw Material | Suppliers | Contact Information |
Phosphoramidites | Thermo Fisher Scientific |
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Glen Research |
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TriLink BioTechnologies |
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BOC Sciences |
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Hongene Biotech Corporation |
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5 Oligonucleotide Pool Library Distributors List
In the field of distribution of oligonucleotide pool library, a group of specialized distributors play the role of a bridge connecting manufacturers and customers. These distributors not only ensure the wide distribution of products, but also enhance the efficiency and responsiveness of the supply chain by providing professional technical support and customized services. Their global network coverage enables oligonucleotide pool library to quickly reach researchers and corporate users around the world, meeting the demand for high-quality biotechnology products in different regions. The expertise and market penetration of these distributors have a significant impact on promoting the global adoption of oligonucleotide pool library technology and advancing related scientific discoveries. By working with these distributors, manufacturers of oligonucleotide pool libraries are able to expand their market coverage while ensuring that their customers receive timely technical support and excellent after-sales service.
Table Oligonucleotide Pool Library Distributors List
Distributors | Contact Information |
BioCat GmbH |
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2BScientific |
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Nordic Biolabs AB |
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e-nnovation Life sciences |
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Life Science AP Company Limited |
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Adelab Scientific |
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6 Oligonucleotide Pool Library Customers
The Major Customers of Global Oligonucleotide Pool Library Market Including: Azenta Life Sciences, CD Genomics, Daicel Corporation, Boster Bio, ProteoGenix, Biomatik.
Table Major Customers of Oligonucleotide Pool Library with Contact Information
Customers | Contact Information |
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Daicel Corporation |
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Boster Bio |
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ProteoGenix |
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7 Oligonucleotide Pool Library Market Trends
Table Market Trends
Item | Descriptions |
Low cost, high precision, automation | The cost to synthesize a whole genome is still very high due to the chemical reagent consumption during oligo synthesis, as well as sequencing validation and error correction steps during oligo assembly steps. The large-scale oligo synthesis technology will be improved toward the aspects of low cost, high accuracy, and longer length. This will achieve a cheaper source of oligonucleotide. To reduce the total cost fundamentally, all the functions, e.g., oligo synthesis, DNA assembly, and DNA analysis, that are required by whole genome synthesis should be integrated into one platform by using modern automation technologies, to accomplish the full function of data writing, copying, reading, and random access in one device. As the underpinning chemistry for synthetic DNA is unlikely to change markedly, the elongation cycle efficiency remains the main limiting factor. This has prompted companies to develop complementary capabilities such as highly parallelized, miniaturized, and automated synthesis while promoting user autonomy in producing DNA. As a result, it will be even more important that the according bioinformatics tools are developed to design DNA. Further, computational tools such as artificial intelligence (AI) and de novo protein design strategies currently revolutionize protein science. For example, AI has been used to guide and accelerate the pace of directed evolution and has recently been used to predict from mostly database‐available sequences which combination of mutations likely yields a functionally optimized protein or enzyme. TargetRanch is a software system developed by Deep Genomics that uses artificial intelligence (AI) predictors trained on large-scale genomics datasets to identify disease causing mutations – and oligonucleotide therapeutics that could treat the resulting problem. |
Oligonucleotides are used to store digital data | As the amount of data generated in the digital age continues to grow exponentially, there is a growing need for storage media with significantly higher information density, durability, and energy costs. Current optical and magnetic device storage media have reached their information density limits and are not suitable for long-term (greater than 50 years) storage. DNA is one of the most promising next-generation data carriers. Theoretical calculations predict synthetic DNA can last in permafrost for up to 28,000 years. It also takes up significantly less space. 1 gram of DNA can store up to a zettabyte of digital data. To put that into perspective, it would only take 20 grams of DNA to store all of the data in the modern world. Additionally, if preserved under optimal conditions and dehydrated, DNA may be preserved for millions of years, making it useful for data storage. Numerous space experiments on microorganisms have also demonstrated their extraordinary durability in extreme conditions, suggesting that DNA could become a durable data storage medium. DNA oligonucleotides take up very little physical space and are stable for thousands of years, making them ideal for long-term data storage. To extract data stored this way, oligonucleotides are run on DNA sequences. This sequence can then be decoded back into binary digital data. Both encoding (writing millions of oligonucleotides) and decoding (DNA sequencing) steps are already present. Recent collaborations have developed ways to store data as DNA sequences. Through relatively simple chemistry, scientists can encode A, T, C, and G nucleotides in oligos in any order, mimicking and expanding on the binary (ones and zeros) data language. Challenges remain, such as the need to improve methods for rapid, error-free synthesis of oligonucleotides. In the long term, researchers will need to develop affordable data storage solutions and will need to improve methods for synthesizing long DNA strands. Methods for reading nucleotide sequences will evolve towards higher confidence. |
Green synthesis technology | Current industrial DNA synthesis processes usually start with chemically synthesized oligonucleotides, and longer DNA molecules are gradually spliced and assembled through enzymatic reactions using oligonucleotides as raw materials. In addition to the synthesis and assembly steps, the production process also includes multi-step product isolation, purification, and detection steps. Green chemistry aims to reduce and eliminate pollutants from the source, improve resource utilization, and reduce energy consumption. Some feasible solutions include: reducing reaction raw materials by increasing monomer conversion rate and reducing loss rate while ensuring output Dosage; recycling and regenerating unreacted raw materials, solvents, catalysts, etc.; actively searching for complete alternatives for raw materials that cannot be recycled, regenerated, and reused and have obvious toxic side effects and pollution effects; develop high-efficiency, high-selectivity, reaction Catalysts with mild conditions and environmental friendliness, such as biological enzymes; further optimization of related carriers such as synthesis and purification; use of computer-aided design and simulation to optimize reaction routes and promote simplification of production steps, etc. Strategies with more sustainable potential include developing miniaturized and parallel reaction systems, maximizing synthesis efficiency to obtain longer DNA, and integrating multiple technologies to improve the scalability of the synthesis platform. |
